We report the design, synthesis, and assembly of the 1.08-Mbp Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a Mycoplasma capricolum recipient cell to create new Mycoplasma mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication. |
“John Craig Venter (born October 14, 1946) is an American biologist and entrepreneur, most famous for his role in being one of the first to sequence the human genome[1] and for his role in creating the first synthetic cell in 2010.[2] Venter founded Celera Genomics, The Institute for Genomic Research and the J. Craig Venter Institute, now working at the latter to create synthetic biological organisms and to document genetic diversity in the world’s oceans. He was listed on Time Magazine’s 2007 and 2008 Time 100 list of the most influential people in the world.” (wikipedia)
Just to keep things in perspective:
“Some other scientists said that aside from assembling a large piece of DNA, Dr. Venter has not broken new ground. “To my mind Craig has somewhat overplayed the importance of this,” said David Baltimore, a geneticist at Caltech. He described the result as “a technical tour de force,” a matter of scale rather than a scientific breakthrough.
“He has not created life, only mimicked it,” Dr. Baltimore said.
Dr. Venter’s approach “is not necessarily on the path” to produce useful microorganisms, said George Church, a genome researcher at Harvard Medical School. Leroy Hood, of the Institute for Systems Biology in Seattle, described Dr. Venter’s report as “glitzy” but said lower-level genes and networks had to be understood first before it would be worth trying to design whole organisms from scratch.
In 2002 Eckard Wimmer, of the State University of New York at Stony Brook, synthesized the genome of the polio virus. The genome constructed a live polio virus that infected and killed mice. Dr. Venter’s work on the bacterium is similar in principle, except that the polio virus genome is only 7,500 units in length, and the bacteria’s genome is more than 100 times longer.”” (The New York Times)
• May 22, 2010 link notes tagged: technology human life cell genome genetic biology replication self-replication science news synthetic